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Image Search Results
Journal: Molecular Endocrinology
Article Title: Advanced Glycated End-Products Affect HIF-Transcriptional Activity in Renal Cells
doi: 10.1210/me.2013-1036
Figure Lengend Snippet: A–C, AGE-BSA modulates HIF-transcriptional activity in renal cells as detected with reporter gene assay analysis. Luciferase activity was normalized to β-galactosidase activity and presented as percentage relative to Co-BSA-treated cells. A, HIF reporter activity in differentiated podocytes. **, P < .001 (n = 18). B, HIF reporter activity in MTC cells. **, P < .001 (n = 18). C, HIF reporter activity in MMCs. *, P < .005 (n = 12). HIF-transcriptional activity was significantly stimulated in differentiated podocytes and MTCs treated with AGE-BSA, whereas it was suppressed in MMCs.
Article Snippet: The luciferase activity was assayed using the Luciferease reagent (Promega) and the β-galactosidase activity was measured via
Techniques: Activity Assay, Reporter Gene Assay, Luciferase